Fig. 1.

Identification of five distinct cell populations in atherosclerotic aorta. (A) Schematic overview of experimental design. (B, C) Uniform manifold approximation and projection (UMAP) visualization of single cells from normal (n = 1063) and atherosclerotic (n = 991) aortae of 3 ApoE^−/− mice representing (B) cellular origin and (C) segregation into 5 distinct clusters. (D) Dotplots demonstrating the gene signatures of the 5 aortic cell types. Dot colors, expression levels; size, cell proportion with expression. EC, endothelial cell; FB, fibroblast; MP, macrophage; ND, normal diet; RBC, red blood cell; VSMC, vascular smooth muscle cell; WD, western diet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)