Fig. 3.

EC subpopulations in atherosclerotic aorta. (A, B) Uniform manifold approximation and projection (UMAP) plots on (A) cellular origin and (B) partition into two subpopulations (EC1 and EC2) of single ECs from normal and atherosclerotic aortae. (C) Dot plots showing the gene signatures of the two EC subpopulations. Dot colors, expression levels; size, cell proportion with expression. (D, E) Violin plots on the expression of selected (D) endothelial markers and (E) mesenchymal markers of 2 EC subpopulations. Dot: median. (F) Immunofluorescence staining on PRSS23 level in cross sections of ApoE^−/− mouse aortae (n = 4 per group; Scale bar: 200 μm). EC, endothelial cell; ND, normal diet; WD, western diet.