Fig. 3.

iNOS inhibition improved Akt-mediated insulin signaling. (A-B) Representative immunostaining and semi-quantification of phosphorylated Akt (Ser473 site) in heart tissue samples. n = 6 per group. (C) Glucose uptake in isolated cardiac myocytes from adult mice. n = 6 per group. (D-E) Representative immunostaining and semi-quantification of phosphorylated Akt at Ser473 site in AC16 human cardiomyocyte cells transfected with iNOS or mutated Akt plasmids. (F) Glucose uptake in AC16 human cardiomyocyte cells transfected with iNOS or mutated Akt-containing plasmids. n = 4 per group. The data are shown as mean ± SEM and were analyzed using one-way ANOVA followed by Tukey’s post hoc test (A-C), or two-way ANOVA with Bonferroni post hoc test (D-F). *, P < 0.05. **, P < 0.01. ***, P < 0.0005. ****, P < 0.0001. ns, no significant.