Fig. 5.

iNOS induces oxidative stress and mitochondrial injury in cardiomyocyte. (A-B) Representative immunostaining and semi-quantification of NOX4 expression in neonatal rat cardiomyocytes transfected with plasmids encoding iNOS. n = 6. (C-D) The mitoSOX was used as an mitochondrial ROS indicator and assessed by flow cytometry. (E-F) The TMRE was used as an mitochondrial membrane potential indicator and assessed by flow cytometry. n = 4. The data are shown as mean ± SEM and were analyzed using one-way ANOVA followed by Tukey’s post hoc test. *, P < 0.05. **, P < 0.01. ***, P < 0.0005. ****, P < 0.0001. ns, no significant.