Fig. 5.

Intrathecal overexpressing GPX4 rescued motor performance disorder in G93A mice. a Representative fluorescence co-localization images of GPX4 and NeuN in the lumbar ventral horn. Scale = 50 μm. N = 3. b Expression of GPX4, ChAT, and 4HNE in the spinal cord of mice. N = 3. c Volcano plot of oxidative phospholipids represented by log₂ (fold change) of G93A + GPX4-AAV/G93A + AAV groups plotted against the -log₁₀ (p-value). Statistical significance was evaluated by t test (p < 0.05). d Amount of PE(38:4) + 2O in the spinal cord of mice. N = 6. e-g Persistence time on the rotating rod, suspension time on the wire mesh, and statistics of neurological scores of mice intrathecally injected with GPX4-AAV. Data are represented as mean ± SEM. N = 6 mice each group, ^***p < 0.001 G93A + AAV vs. WT + AAV group; ^###p < 0.05 G93A + GPX4-AAV vs. G93A + AAV group by Two-way ANOVA. h Representative Nissl staining images and quantification of motor neurons in the lumbar VH. N = 6. i Representative images and the integrated fluorescence density of NeuN-stained motor neurons in the lumbar VH at the terminal age. N = 5. j Representative images and the integrated fluorescence density of GFAP-stained astrocytes in lumbar VH at the terminal age. Scale = 100 μm. N = 6 mice each group. k Pan (Gfap, Steap4), A1 (Serping1, Utg1a) and A2 (Clcf1, S100a10) astrocyte genes expression in the spinal cord of mice detected by qPCR, and expressed using a heatmap. N = 3. Data are expressed as mean ± SEM, ^**p < 0.01, ^***p < 0.001 G93A + AAV vs. WT + AAV group; ^#p < 0.05, ^###p < 0.001 G93A + GPX4-AAV vs. G93A + AAV group by One-way ANOVA.