TABLE 1.
Neutralization of Stx
| E. coli strain | % Stx neutralizeda
|
||
|---|---|---|---|
| Stx1 | Stx2c | Stx2e | |
| CWG308 | 0 | 0 | 0 |
| CWG308:pJCP-Gb3 | 99.9 | 99.2 | 98.4 |
| CWG308:pJCP-lgtCDE | 99.9 | 98.4 | 98.4 |
| CWG308:pJCP-lgtCDE/wbnF | 99.8 | 98.4 | 98.4 |
| CWG308:pJCP-lgtCDE/gne | 96.9 | 87.5 | 98.4 |
Toxin neutralization was assayed using Vero cells as described in the text. Assays were performed in duplicate with identical results. Untreated toxin extracts had endpoint titers of 656,000, 164,000, and 62,000 CD/ml for Stx1, Stx2c, and Stx2e, respectively.