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. Author manuscript; available in PMC: 2023 Jan 4.
Published in final edited form as: J Neurochem. 2020 Sep 17;158(4):943–959. doi: 10.1111/jnc.15157

FIGURE 3.

FIGURE 3

Effect of acute stress on CRF1, CRF, and CRFBP expression in the prefrontal cortex of male and female mice. Mice were exposed to 2-hr-restraint stress and sacrificed 1.5 hr after the end of the stress procedure. (a) As reported in this graph, CRF1 expression significantly increases in both males (black bars) and females (white bars) at the age of 1, 6, 12, and 18 months following acute stress. (b) CRF levels significantly increase exclusively in 1-month-old stressed males. (c) CRFBP expression changes only in 18-month-old male mice under stress conditions. (d) Representative bands for CRF1, CRF, CRFBP, and β-actin. m CO, male controls; f CO, female controls; m ST, male stress; f ST, female stress. Values are reported in percentage; average of control male mice is considered as 100%. CRF1: 51 kDa; CRF: 18 kDa; CRFBP: 37 kDa; β-actin: 43 kDa. Data represent the mean ± SEM of 5 mice. *p < .05, **p < .01, and ***p < .001 when compared with control male mice; #p < .05, ##p < .01, and ###p < .001 when compared with control female mice. Two-way ANOVA followed by Tukey's post hoc analysis