Five compounds selected from the docking library inhibit
the aggregation
of α-synuclein. (A) Kinetic profiles of a 10 μM solution
of α-synuclein in the presence of 25 nM seeds at pH 4.8 and
37 °C, in the presence of 1% DMSO alone (beige), in the presence
of 10 molar equivalents of compounds A–E (represented in different
colors), or in the presence of 10 molar equivalents of other compounds
in the docking library that did not affect significantly α-synuclein
aggregation (black). (B) Relative t1/2 of the aggregation of α-synuclein in the presence of compounds
A–E as shown in (A), normalized to the DMSO control. (C) Chemical
structures of compounds A–E. Throughout, error bars represent
mean ± SEM of two replicates.