Extended Data Fig. 6. Impact of SuCas12a2 guide and PFS alterations on plasmid targeting in E. coli BL21(AI).

a, Impact of guide length on plasmid clearance. The guide sequence is depicted with blue letters. The standard crRNA guide length based on crRNA processing (Extended Data Fig. 3) is 24 nts. b, Reduction in plasmid transformation by SuCas12a2 and LbCas12a in the presence of a shared PAM/PFS, Cas12a2-specific PFS, and mismatches to the target under target plasmid and nuclease plasmid or nuclease plasmid-only selection. Values are means ± s.d. of at least 3 independent experiments started from separate colonies. ns: p > 0.05, *: p < 0.05, **: p < 0.005 calculated with one-tailed Welch’s t-test.