Extended Data Fig. 7. SuCas12a2 degrades cellular RNA.

a, 1% agarose gel resolving total RNA extracted from E. coli BL21(AI) expressing SuCas12a2, LbCas12a and LsCas13a under target (T) and non-target (NT) conditions. Expression of the nucleases and crRNA was induced with 10 nM IPTG and 0.2% arabinose for 2 h. Individual wells for each condition represent biological replicates. Nucleotide sizes are based on a resolved RNA ladder. sRNA pool: RNA pool comprising tRNAs and other small RNAs. b, Quantification of band intensities across the 4 independent experimental replicates started from separate colonies. Significance was calculated using two-tailed Welch’s t-test (ns: p > 0.05, *: p < 0.05, **: p < 0.005).