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Sensitivity of Pseudoloma neurophilia ddPCR by evaluation of inoculated filters in duplicate. Two separate experiments with starting concentrations of (A) 100,000 spores/L or (B) 77,500 spores/L. Each DNA assay was conducted in triplicate, with copy number reported for each PCR technical replicate at each concentration. Circles represent triplicate PCR reactions for filter 1 (F1), and squares represent the triplicate PCR reactions for filter 2 (F2).
