Table I.

Summary of longitudinal evaluation of 5 tanks of zebrafish with Pseudoloma neurophilia infections. Each cell represents results, positive (+) or negative (−) following ddPCR testing of 2 (1 L) water samples taken from a tank with flowing water (flow), with water turned off for 8 hr (static) or water from a group spawn (spawn). DNA extracts from each filter sample were evaluated in triplicate. Number of positive replicates/tanks of the 6 replicates are in parentheses in each cell (No.). A positive score for a tank sample occurred when at least 1 of the 2 L samples showed detection of 2 or more replicates. Eggs from successful spawns were collected, counted, and equally divided into 10% pools and tested. Unless the spawns produced a small number of eggs, such as tank 1 in November and tank 2 in January, which has a total of 6 and 5 eggs (respectively), they were grouped into 1 pool. All egg samples were negative. Neural tissues from fish were evaluated by qPCR after the last water and spawn samples were collected.

Tank No.	Flow			Static			Spawn			Total No. of eggs			% Positive tank samples	Tissue samples positive by QPCR (%)
	Nov	Dec	Jan	Nov	Dec	Jan	Nov	Dec	Jan	Nov	Dec	Jan
Tank 1	− (1)	+ (3)	− (0)	− (2)	+ (4)	+ (3)	+ (3)	+ (5)	+ (5)	6	0	0	(67)	(54)
Tank 2	− (1)	+ (6)	+ (3)	+ (6)	+ (6)	+ (6)	+ (4)	+ (3)	+ (5)	159	83	5	(89)	(83)
Tank 3	− (1)	+ (3)	− (1)	− (1)	+ (5)	− (1)	+ (4)	+ (4)	+ (4)	0	0	90	(56)	(42)
Tank 4	+ (3)	+ (6)	− (2)	+ (3)	+ (5)	+ (5)	− (2)	+ (5)	− (1)	0	30	360	(67)	(68)
Tank 5	+ (3)	− (1)	− (1)	+ (3)	+ (6)	+ (6)	− (0)	+ (4)	+ (5)	0	80	72	(67)	(63)
% positive by sampling method	(47)			(80)			(80)