Figure 1.

In vitro validation of the efficacy of cell-penetrating, AR-targeting asiRNA (cp-asiAR) knockdown. (A) A549 cells were treated with cp-asiARs at indicated concentrations by passive uptake. mRNA levels of AR observed 24 h after treatment were normalized using tubulin mRNA expression. AR mRNA levels relative to those of vehicle control (VC) are presented as mean and standard deviation (n = 6). AR protein levels at 48 h after treatment are shown below. β-actin protein level was considered as a loading control. (B) Knockdown efficacy was validated in T98G cells. Representative images are shown. NTC denotes nontargeting control siRNA treatment at a concentration of 10 μM. Statistical significance was calculated using a t-test with VC (*, p < 0.05, **, p < 0.01, ***, p < 0.001).