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. 2022 Jul 1;43(17):5220–5234. doi: 10.1002/hbm.25998

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© 2022 The Authors. Human Brain Mapping published by Wiley Periodicals LLC.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Flow chart of the method used. First, we used preprocessed diffusion magnetic resonance imaging (dMRI) data to extract features for segmenting the cortex into layers. Then, we added generalized fractional anisotropy to the features and segmented again. The results obtained before and after adding generalized fractional anisotropy were compared and analyzed. Furthermore, the results were compared with histological data, with the superficial layer corresponding to Brodmann's layers 1–3 and the deep layer corresponding to layers 4–6. Finally, the results were used for the estimation of laminar connections and for laminar analysis of the working memory.