Fig. 4. Alternative splicing analysis.

(A and B) The sixth exon in MYL6 is skipped at different proportions in different compartments. Cells in the immune and epithelial compartments tend to skip the exon, whereas cells in the endothelial and stromal compartments tend to include the exon. Boxes are grouped by compartment and colored by tissue. The fraction of junctional reads that include exon 6 was calculated for each cell with >10 reads mapping to the exon-skipping event. Horizontal box plots in (B) show the distribution of exon inclusion in each cell type. (C and D) Alternative splicing in CD47 involves one 5′ splice site (exon 11; 108,047,292) and four 3′ splice sites. Horizontal box plots in (D) show the distribution of weighted averages of alternative 3′ splice sites in each cell type. Epithelial cells tend to use exons closer to the 5′ splice site compared with immune and stromal cells. Boxes are grouped by compartment and colored by tissue.