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Alignments of TaPIN1 nucleotide (A) and predicted amino acid (B) sequences. Sequences derived from a buparvaquone resistant Tunisian isolate and various Turkish T. annulata isolates with treatment failure history were compared for mutations with the T. annulata / C9 reference genome, obtained from PiroplasmDB.org database; the systematic identifier for TaPIN1 is TA18945. Nonsynonymous substitutions detected among alignments were indicated with an asterisk (*) coloured in yellow.
