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. 2023 Jan 4;42(1):111964. doi: 10.1016/j.celrep.2022.111964
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
VH-FC ab8 (Li et al.)18 N/A
Fab S309 (Pinto et al.)20 N/A
Fab S2M11 (Tortorici et al.)19 N/A
Fab 4-8 (Liu et al.)21 N/A
Fab 4A8 (Chi et al.)22 N/A
Fab DH1052 (Li et al.)23 N/A
Goat anti-human IgG Jackson ImmunoReserach Cat. # 109-035-088; RRID: AB_2337584
Mouse anti-his tag antibody abcam Cat# ab18184; RRID: AB_444306
Chemicals, peptides, and recombinant proteins
Ace2 (18-615) New England Biolabs Cat. # 73775S
BA.1 RBD Sino Biological Cat# 40592-V08H121
Critical commercial assays
Pierce 1-Step Ultra Substrate Solution ThermoFisher Cat. # 34028
Deposited data
S(BA.2) This paper Global refinement: EMDB 27523, PDB: 8DM1
Focus refinement: EMDB 27524, PDB: 8DM2
S(BA.2)+hACE2 This paper Global refinement: EMDB 27527, PDB: 8DM5
Focus refinement: EMDB 27528, PDB: 8DM6
S(BA.2)+mACE2 This paper Global refinement: EMDB 27529, PDB: 8DM7
Focus refinement: EMDB 27530, PDB: 8DM8
S(BA.1)+mACE2 This paper Global refinement: EMDB 27531, PDB: 8DM9
Focus refinement: EMDB 27532, PDB: 8DMA
S(BA.2)+4A8 This paper Global refinement: EMDB 27525, PDB: 8DM3
Focus refinement: EMDB 27526, PDB: 8DM4
Experimental models: Cell lines
Expi293F ThermoFisher Cat# A14527
Recombinant DNA
pcDNA3.1 HexaPro BA.1 Mannar et al.9 N/A
pcDNA3.1 BA.1 NTD This paper N/A
pcDNA3.1 HexaPro BA.2 This paper N/A
pcDNA3.1 BA.2 NTD This paper N/A
pcDNA3.1 BA.2 RBD This paper N/A
pcDNA3.1 hACE2 (1-615) Mannar et al.9 N/A
pcDNA3.1 mACE2 (1-615) This paper N/A
pCSCG hACE2-FC Yang et al.39 Addgene: 164222
pcDNA3.1 Fab S309 Light Chain Mannar et al.30 N/A
pcDNA3.1 Fab S309 Heavy Chain Mannar et al.30 N/A
pcDNA3.1 Fab S2M11 Light Chain Mannar et al.30 N/A
pcDNA3.1 Fab S2M11 Heavy Chain Mannar et al.30 N/A
pcDNA3.1 Fab 4-8 Light Chain Saville et al.40 N/A
pcDNA3.1 Fab 4-8 Heavy Chain Saville et al.40 N/A
pcDNA3.1 Fab 4A8 Light Chain Saville et al.40 N/A
pcDNA3.1 Fab 4A8 Heavy Chain Saville et al.40 N/A
pcDNA3.1 Fab DH1052 Light Chain This paper N/A
pcDNA3.1 Fab DH1052 Heavy Chain This paper N/A
Software and algorithms
GraphPad Prism GraphPad 7.0 https://www.graphpad.com/scientific-software/prism/
EPU automated acquisition ThermoFisher Scientific https://www.thermofisher.com/us/en/home/electron-microscopy/products/software-em-3d-vis/epu-software.html
UCSF Chimera Pettersen et al.41 https://www.cgl.ucsf.edu/chimera/
UCSF ChimeraX Goddard et al.42 https://www.cgl.ucsf.edu/chimerax/
RELION 3.1 Scheres43 https://github.com/3dem/relion/releases/tag/3.1.0
cryoSPARC live (v3.0.1) Punjani et al.44 https://cryosparc.com/live
COOT Emsley et al.45 https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
Phenix Liebschner et al.46 https://phenix-online.org/
MolProbity Williams et al.47 http://molprobity.biochem.duke.edu/
PyMOL v.2.2 Schrodinger, LLC https://pymol.org/2/