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. Author manuscript; available in PMC: 2023 Jun 2.
Published in final edited form as: Cancer Discov. 2022 Dec 2;12(12):2930–2953. doi: 10.1158/2159-8290.CD-21-0865

Fig. 3. Single-cell transcriptional profiling reveals alveogenic mimicry.

Fig. 3.

A, UMAP plot showing mammary epithelial cells from control, Pik3caH1047R and Pik3caH1047R;Kdm6afl/fl mutant mice 2 weeks after Ad-Cre injection. B, Dot Blot showing differentially expressed marker genes within the different epithelial lineages stratified by genotypes. C, Pathways differentially enriched in Pik3caH1047R;Kdm6afl/fl versus control and Pik3caH1047R;Kdm6afl/fl versus Pik3caH1047R mammary epithelial LP cells identified using g:Profiler (p <0.05 with Benjamini-Hochberg FDR correction, > 10-fold enrichment). The top 20 enriched pathways are shown. Heat- map depicts how these pathways are altered in the major 3 epithelial lineages. D, UMAP and violin blots showing alveogenesis signature. E, Immunohistochemistry of mammary glands 2 weeks post injection stained with anti-β-Casein. Scale bar is 100 μm.