. 2022 Dec 28;2022:1040650. doi: 10.1155/2022/1040650

Table 1.

List of selected SNPs of Leptin gene investigated in this study and the respective primers used for amplification of the regions of interest.

Primer ID No.	SNP/db SNP ID	Region	Primers (5′—3′)	Tm	Flanking sequences
1	−2548A/G rs7799039	Promoter-1	F-TGGGCATCCCCACAGACGGA	75.4	AGGGTTGC[A/G]CTGATCCT
1	−2548A/G rs7799039	Promoter-1	R-TCACAGTGGTCCTGAGGTGACG	69.9	AGGGTTGC[A/G]CTGATCCT

2	−633C/T rs28954080	5′-UTR	F-CACGCCTGGCTGGGTTGGTT	73.5	CCAGAGAG[C/T]GTGCACTC
2	−633C/T rs28954080	5′-UTR	R-TGCGGGGTTGGGGGTTCTGA	75.9	CCAGAGAG[C/T]GTGCACTC

3	−188C/A rs791620	Exon-1	F-TCGCTCCTACCAGCCACCCC	72.7	GCTCCTGG[A/C]GCGCCGAG
3	−188C/A rs791620	Exon-1	R-GTCCTGCCTTCTGGGCACCG	73.4	GCTCCTGG[A/C]GCGCCGAG

3	+19G/A rs2167270	Exon-1	F-TCGCTCCTACCAGCCACCCC	72.7	CAGCGCCA[A/G]CGGTTGCA
3	+19G/A rs2167270	Exon-1	R-GTCCTGCCTTCTGGGCACCG	73.4	CAGCGCCA[A/G]CGGTTGCA

4	+45G/A rs145044661	Exon-2	F-TGAGGGGATGGTAGCCAGAGCA	72.0	TCAATGAC[A/G]TTTCACAC
4	+45G/A rs145044661	Exon-2	R-TCAGCAGGCAGAGGGGCCAA	74.6	TCAATGAC[A/G]TTTCACAC

5	+102C/T rs28954113	Exon-3	F-GGCTCCACCCCATCCTGACCT	72.6	CTGGAGAA[A/C/G/T]CTCCGGGAT
5	+102C/T rs28954113	Exon-3	R-TGCTGGGTGGACCCCCTTCC	74.6	CTGGAGAA[A/C/G/T]CTCCGGGAT

5	+144A/G rs1800564	Exon-3	F-GGCTCCACCCCATCCTGACCT	72.6	CTTCTTCAC[A/G/T]TGCTGGCC
5	+144A/G rs1800564	Exon-3	R-TGCTGGGTGGACCCCCTTCC	74.6	CTTCTTCAC[A/G/T]TGCTGGCC

5	+328G/A rs28954114	Exon-3	F-GGCTCCACCCCATCCTGACCT	72.6	AGGACTAC[A/G]TTAAGGGA
5	+328G/A rs28954114	Exon-3	R-TGCTGGGTGGACCCCCTTCC	74.6	AGGACTAC[A/G]TTAAGGGA