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. 2023 Jan 1;36(1):60–71. doi: 10.3344/kjp.22277

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© The Korean Pain Society, 2023

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4 — Heparanase (HPSE) up-regulates nerve growth factor (NGF) and is promoted by NGF feedback. (A) The expression of NGF in the model and control mice was detected by western blot. (B, C) The expression of NGF was detected by western blot and qRT-PCR after HPSE treatment. ^**P < 0.01 versus control. (D) The expression of NGF was determined by qRT-PCR after NGF treatment. ^**P < 0.01 versus vector. (E) The expression of NGF was detected by qRT-PCR after NGF silencing. ^**P < 0.01 versus si-NC. (F) The expression of HPSE was detected by qRT-PCR after NGF treatment. ^**P < 0.01 versus vector. (G) The expression of HPSE was detected by qRT-PCR after NGF silencing.^**P < 0.01 versus si-NC. The error bars indicate mean ± standard deviation. qRT-PCR: quantitative real-time polymerase chain reaction, si-NC: siRNA negative control.