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Primers for qRT-PCR in this study
| Gene | Forward | Reverse |
|---|---|---|
| HPSE | 5’-AGACGGCTAAGATGCTGAAGAG-3’ | 5’-TCTCCTAACCAGACCTTCTTGC-3’ |
| NGF | 5’-CAGGACTCACAGGAGCAAGC-3’ | 5’-GCCTTCCTGCTGAGCACACA-3’ |
| PGE2 | 5’-CGGTGATGTTCATCTTCGG-3’ | 5’-GTAGGCGTGGTTGATGGC-3’ |
| β-actin | 5’-AGCCATGTACGTAGCCATCC-3’ | 5’-CTCTCAGCAGTGGTGGTGAA-3’ |
qRT-PCR: quantitative real-time polymerase chain reaction, HPSE: heparanase, NGF: nerve growth factor, PGE2: prostaglandin E2.
