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(a) Confocal images of the co-localization of fusion protein PD-1-mCherry and DiO-labelled cell membrane. Scale bar: 5 μm; (b) flow cytometry analysis of mCherry positive rate of CHO-PD1 cells; (c) western blot analysis of PD-1 expression on CHO–S and CHO-PD1 cells, and β-actin was used as control.
