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. 2022 Dec 21;613(7942):169–178. doi: 10.1038/s41586-022-05535-x

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© The Author(s) 2022, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a, SPiDER⁺ and SPiDER⁻ populations from 3 d.p.i. muscle stained with Oil Red O and haematoxylin. Images and lipid-droplet quantification. n = 90 SCs (NSen), 51 SCs (Sen), 89 FAPs (NSen), 90 FAPs (Sen), 45 MCs (NSen) and 94 MCs (Sen). b, Heat map showing lipid-transport-related genes that are differentially expressed in at least 3 out of 12 comparisons between Sen and NSen cells. The colour indicates the log₂-transformed fold change in expression. c, Images and CD36 quantification in SPiDER⁺ and SPiDER⁻ populations from 3 d.p.i. muscles. n = 44 SCs (NSen), 50 SCs (Sen), 56 FAPs (NSen), 62 FAPs (Sen), 55 MCs (NSen) and 56 MCs (Sen). d, SA-β-gal⁺ cells in the injured TA area of aged mice (treated with anti-CD36 antibodies or control IgA). n = 8 TA from 4 mice for both groups. e, The overlap between SASP-upregulated genes in 7 d.p.i. senescent populations of old mice, and those reduced by anti-CD36 treatment. f, The CSA and frequency distribution of MYH3⁺ fibre size (n = 6 TA from 4 mice for both groups) and Sirius Red staining (n = 8 TA from 4 mice for both groups) of 7 d.p.i. TA from old mice treated with anti-CD36-antibody antibodies or control IgA. g, Injured EDL of old mice treated with anti-CD36 antibodies or control IgA. (n = 6 EDL from 3 mice (IgA) and n = 5 EDL from 4 mice (anti-CD36)). Force–frequency curves are shown. h, SPiDER⁺ and SPiDER⁻ cells from 3 d.p.i. muscles were transfected with siCd36 or siScramble, stained with Dil and transplanted into the preinjured recipient TA. Images and the CSA of MYH3⁺ fibres 4 days after transplantation. n = 6 (siScramble-treated SPiDER⁺ and SPiDER⁻ cells) and n = 4 (siCd36-treated SPiDER⁺ and SPiDER⁻ cells) mice. i, SCs (from 3 d.p.i. muscles) were cultured for 3 days in Transwells with senescent or non-senescent C2C12 cells that were previously treated with siCd36 or siScramble, or without cells. BrdU incorporation is shown. n = 4 (empty Transwell) and n = 3 (other groups) mice. Scale bars, 10 μm (a and c) and 50 μm (h). For a, c, d and f–i, data are mean ± s.e.m. P values were calculated using Mann–Whitney U-tests (a, c and f), mixed-effects analysis (g) and Tukey’s tests (h and i); NS, not significant.

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