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. 2022 Dec 14;613(7942):120–129. doi: 10.1038/s41586-022-05534-y

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© The Author(s) 2022, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 10 — a) Images of TGFβR1 expression (green) by OLIG2+ cells (magenta) in corpus callosum of Tgfbr1^fl/fl and Plp-creERT;Tgfbr1^fl/fl mice, who received 4-hydroxy tamoxifen (OHT; 100 mg/kg/day, P14–18) and were sacrificed at P28. Scale bar, 20 µm. b) Mean relative TGFβR1 intensity ± s.e.m. per OLIG2+ cell in Tgfbr1^fl/fl and Plp-creER;:Tgfbr1^fl/fl mice. n = 3 mice/group. *P = 0.011, 2-tailed unpaired Student’s t-test. c) Mean number of myelinated axons/axon diameter bin ± s.e.m. in wildtype mice (WT; green), Plp-creERT;Tgfbr1^fl/fl mice (magenta) and Tgfbr1^fl/fl mice (blue). n = 3 mice/group. Non-significant, P = 0.3001, 2-way ANOVA with Sidak’s multiple comparisons test. d) Mean inner tongue thickness (µm)/axon diameter bin ± s.e.m. in wildtype mice (WT; green), Plp-creERT;Tgfbr1^fl/fl mice (magenta) and Tgfbr1^fl/fl mice (blue). n-3 mice/group. <0.3 µm WT vs. Plp-creERT;Tgfbr1^fl/fl: *P = 0.03950, 2-way ANOVA with Sidak’s multiple comparisons test. e) Mean myelin tongue thickness (µm)/axon diameter bin ± s.e.m. in wildtype mice (WT; green), Plp-creERT;Tgfbr1^fl/fl mice (magenta) and Tgfbr1^fl/fl mice (blue). n = 3 mice/group. <0.3 µm WT vs. Tgfbr1^fl/fl: *P = 0.0291; >1 µm WT vs. Plp-creERT;Tgfbr1^fl/fl **P = 0.0037 and Plp-creERT;Tgfbr1^fl/fl vs. Tgfbr1^fl/fl ***P = 0.0003, 2-way ANOVA with Sidak’s multiple comparisons test. f) Mean number of myelinated axons ± s.e.m./axon diameter bin in 3-month-old FIRE^Δ/Δ mice following treatment with vehicle control or SRI-011381 hydrochloride from 2 to 3 months of age. n = 3 Vehicle-treated and 4 SRI-treated mice. Non-significant, P = 0.9202, 2-way ANOVA with Sidak’s multiple comparisons test. g) Mean inner tongue thickness (µm) ± s.e.m. per axon diameter bin in 3-month-old FIRE^Δ/Δ mice following treatment with vehicle control or SRI-011381 hydrochloride from 2 to 3 months of age. n = 3 Vehicle-treated and 4 SRI-treated mice. 0.5-1.0 µm: *P = 0.0334, >1 µm: ****P < 0.0001, 2-way ANOVA with Sidak’s multiple comparisons test. h) Mean myelin thickness (µm) ± s.e.m./axon diameter bin in 3-month-old FIRE^Δ/Δ mice following treatment with vehicle control or SRI-011381 hydrochloride from 2 to 3 months of age. n = 3 Vehicle-treated and 4 SRI-treated mice. 0.5-1.0 µm: **P = 0.0027, >1 µm: ****P < 0.0001, 2-way ANOVA with Sidak’s multiple comparisons test.

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