FIG. 11.

MDP and LPS up-regulated MyD88 mRNA expression in THP-1 cells. OCT-differentiated THP-1 cells were treated with MDP (10 μg/ml), S. abortus-equi LPS (10 ng/ml), and MDP plus LPS for 2 to 24 h. Total cellular RNA (20 μg/lane) extracted from the cells was electrophoresed, transferred to a nylon membrane, and then analyzed by Northern blotting using a ³²P-labeled cDNA probe for MyD88. Relative induction of MyD88 mRNA expression were determined with a BAS 1500 Mac image analyzer (Fuji Photo Film) after normalization using GAPDH. The results are representative of two different experiments.