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. Author manuscript; available in PMC: 2024 Jan 1.
Published in final edited form as: Bioorg Med Chem. 2022 Dec 1;77:117112. doi: 10.1016/j.bmc.2022.117112

Table 3.

Binding parameters for GA-NMM complexes obtained via UV-Vis and fluorescent titrations in 5K buffer. Graphical representation of this data can be found in Figure S10.

GA1 GA2 GA3
Method UV-vis FL UV-vis FL UV-vis FL
Red Shift, nm 21.0 ± 0.5 - 21.3 ± 0.2 - 21.4 ± 0.4 -
Hypochromicity, % 20.9 ± 2.4 - 19 ± 4 - 28 ± 6 -
Isosbestic point, nm 395.6 ± 0.6 - 395.6 ± 0.3 - 397 ± 1 -
FL enhancement - 27 ± 4 - 33 ± 16 - 27 ± 4
Ka, μM−1 (1: 1NMM) 0.19 ± 0.05 0.4 ± 0.2 1.4 ± 0.1 1.0 ± 0.2 0.24 ± 0.03 0.4 ± 0.2
Ka, μM−1 (1:2NMM) 0.07 ± 0.02 0.18 ± 0.07 - 0.36 ± 0.03 0.095 ± 0.01 0.2 ± 0.1
Ka, ÅM−1 (2:1NMM) 0.8 ± 0.2 1.1 ± 0.6 - 3.7 ± 1.4 0.96 ± 0.01 1.1 ± 0.8