Figure 5).

Semi-quantitative evaluations of glycopeptide dilution series across temperature. A) Plots demonstrating the linear response of glycopeptide identifications across dilutions. When temperature is held constant, spectral counting may be a viable option for LFQ. B) Distribution of linear regression for quantifiable glycopeptides as temperature increases. While 45°C is most beneficial for glycopeptide identifications, it is not the most optimal for LFQ. Conversely, identifiable glycopeptides may be best quantified at 60°C. C) Change in spectral count as temperature climbs. While PSM count for mannose type glycans is relatively conserved, complex and sialylated glycopeptides show significant differences across temperatures. This observation suggests that LFQ methods may be significantly impacted at different temperatures.