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. Author manuscript; available in PMC: 2023 Jul 4.
Published in final edited form as: Cancer Prev Res (Phila). 2023 Jan 4;16(1):5–16. doi: 10.1158/1940-6207.CAPR-22-0193

Figure 1.

Figure 1.

WA treatment decreased protein levels of ACLY and ACC1 in human breast cancer cells. (A) Representative confocal images (60× oil objective magnification, scale bar = 50 μm) for ACLY and ACC1 proteins (green fluorescence) in MCF-7 or MDA-MB-231 cells following 24-hour treatment with DMSO (control) or WA. Nucleus was stained with DAPI (blue fluorescence). (B) Quantitation of corrected total cell fluorescence (CTCF) using ImageJ software. The results shown are mean ± SD (n = 3). Statistically significant (*P < 0.05) compared with the DMSO-treated control by one-way ANOVA followed by Dunnett’s test. The experiment was repeated two times and the results were consistent.