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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Reproduction. 2023 Jan 4;165(2):183–196. doi: 10.1530/REP-22-0355

Fig. 4. TCFL5 regulates male meiosis I and spermiogenesis.

Fig. 4.

(A) The volcano plot shows the genes whose mRNA abundance increased or decreased significantly in FACS-purified primary spermatocytes from Tcfl5+/em1 heterozygotes (n = 3), compared to C57BL/6 (n = 3).

(B) TCFL5 CUT&RUN peaks at the promoters of Sox30, Rfx2, Foxj1, Dot1l, Ehmt2, and Zfp37. WT denotes C57BL/6.

(C) MEME-reported canonical TCFL5 binding motif underlying the TCFL5 CUT&RUN peaks.

(D) Distance (mean of two trials) from the nearest TCFL5 CUT&RUN peak to the transcription start site (TSS) for three categories of genes: mitosis-specific, meiosis I-specific, and genes whose expression starts at meiosis and persists through spermiogenesis. Genes were classified as not bound by TCFL5 if they had no significant TCFL5 peak ± 500 bp from the transcription start site in any of the CUT&RUN or ChIP-seq datasets. Vertical black lines: median; whiskers: maximum and minimum values, excluding outliers.

(E) STRA8 ChIP-seq and TCFL5 and A-MYB CUT&RUN peaks at the promoters of Tut4/7, Dis3l2, and Kctd19. WT denotes C57BL/6.