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. 2022 Jul 15;44(1):234–243. doi: 10.1038/s41401-022-00949-9

Fig. 4. ROS production participates in the mechanism by which TXF inhibits IRE1α.

Fig. 4

a ROS productions induced by TXF (500 nM) over increasing time and b IRE1α RNase activities over increasing TXF in the presence of reducing reagents DTT (1 mM), DHLA (1 mM), GSH (2 mM), and β-ME (2 mM). c ROS production and d IRE1α RNase activities over increasing TXF in the presence or absence of Trolox (1 mM). e Fluorescence intensity of ROS (FL1-H) probed by flow cytometry and f XBP1 splicing of HeLa cells treated with TXF (1 μM) and TM (1 μg/mL) in the presence or absence of NAC (5 mM) or Trolox (1 mM) for 8 h.