FIG. 5.

RT-PCR transcriptional analysis. Expression of the 10 loci identified was examined in EGD grown in BHI medium. Appropriate pairs of primers internal to each gene were used for RT-PCR. Amplified DNA fragments were detected after electrophoresis in 1% agarose gels by ethidium bromide staining. One microgram of molecular weight DNA ladder (MW; Gibco-BRL) was loaded at both sides of the gel. The fragments were arbitrarily loaded from the largest to the smallest (left to right).