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. 2022 Jun 20;72(1):73–85. doi: 10.1007/s00262-022-03226-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Figure. 5 — IL-10 derived from B10 activates MDSCs through the STAT3 pathway. a MDSCs were isolated from the spleens of the two groups of tumor-bearing mice on day 28 by CD115 magnetic beads and cocultured with mouse MCs isolated by density gradient centrifugation from BAFF WT and BAFF KO mouse spleens at a ratio of 1:3 for 72 h or alone, under the stimulation of LPS (1 µg/ml). The concentration of INOS in the coculture supernatant was detected by ELISA. b The culture supernatant of B^WT, MDSCs cocultured with B^WT, MDSCs cocultured with B.^WT in the presence of IL-10 neutralizing antibody was collected and then cocultured with MDSCs for 48 h. The STAT3 phosphorylation level of MDSCs was detected by western bolt. c Statistical analysis of the STAT3 phosphorylation level of MDSCs. d Mouse MCs isolated by density gradient centrifugation were cocultured with MDSCs at a ratio of 1:3 for 72 h with or without WP1066 or colivelin stimulation. The differentiation ratio of B10 cells was detected by flow cytometry. e Statistical analysis of B10 differentiation in vitro. f Graphical abstract of MDSCs cross-talk with B10 cells by BAFF/BAFF-R pathway to promote immunosuppression in cervical cancer. Data from at least three independent experiments were analyzed using Student’s t test and are expressed as the mean ± SD. Symbols represent statistical significance. (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001)