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. 2022 Nov 22;36(1):209–222. doi: 10.1007/s13577-022-00820-3

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Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Overexpression of GPX4 reversed the effects mediated by TRIM59. A The expression of GPX4 in L02 cells transduced with oeGPX4 lentivirus. B–E L02 cells were treated with 0.4 mM PA, oeGPX4 lentivirus, and oeTRIM59 lentivirus. B Western blot detected the expression of TRIM59 and GPX4. C Oil red O staining detected the level of steatosis in L02 cells (magnification: 200×). D ELISA assay detected the level of TNF-α, IL-6, and IL-8. E Flow cytometry detected the level of lipid ROS. **P < 0.01, ***P < 0.001