Fig. 7.
Knockdown of TRIM59 attenuated HFD-induced steatosis and ferroptosis in NAFLD mice. A The expression of TRIM59 in AML12 cells transduced with shTRIM59 adenovirus. B–F NAFLD mice model was constructed with the application of high-fatty diet. Mice were intravenously treated with shTRIM59 and control adenovirus for 8 weeks. B Western blot detected the expression of TRIM59 and GPX4 in liver tissues of mice. C HE and oil red O staining of liver tissues. D The level of AST, ALT, and TG in NAFLD mice. E ELISA detected the level of TNF-α, IL-6, IL-8 in NAFLD mice. F The level of Fe2+, ferritin, and TRF in NAFLD mice. *P < 0.05, **P < 0.01, ***P < 0.001