Fig. 3.

The size and purity of microglial cells obtained in the F-CM and NM groups. a–c The size of primary microglia isolated from the NM (a) or F-CM (b) groups. n = 3. D–l The purity of primary microglia isolated from the NM (d, g, j) or F-CM (e, h, k) groups was determined by flow cytometry with CD11b and CD45 staining. CD11b (d–f) and CD45 (g–i) were independently analyzed or determined together (j–l) using FlowJo software. n = 5. *p < 0.05; **p < 0.01; ns: no significant difference