Fig. 4.

RT and anti-TIGIT combination therapy improve long-term survival immune memory effect, and abscopal effect. (a-b). The Kaplan Meier survival curve depicts the primary endpoint in mouse models of MC38 and B16 F10 treated with anti-TIGIT therapy, RT, and RT + anti-TIGIT therapy, respectively. c. Ninety days after tumor eradication, the mice were rechallenged with 2 × 10.⁶ MC38 cells on the opposite flank. Both groups did not receive additional treatment. Tumor-free mice that underwent combination therapy were resistant to the tumor rechallenge. d. Combination treatment greatly reduced the growth of secondary tumors. Tumors on the right flank were measured and monitored. Representative data are shown from two experiments conducted with five mice per group. Data are presented as means ± SEM (standard errors of the mean). *p < 0.05; **p < 0.01; ***p < 0.001. RT, radiotherapy; TIGIT, T cell immunoreceptor with immunoglobulin and ITIM (immunoreceptor tyrosine-based inhibitory motif) domains