FIG. 4.

Pretreatment with anti-FAP antiserum fails to block FN binding by M. avium subsp. paratuberculosis. Graded concentrations of NRb IgG (□) or anti-FAP IgG (■) in pH 6 buffer were incubated with acid-treated M. avium subsp. paratuberculosis for 1 h. After unbound antibodies were separated from cells by centrifugation, FN was added to the microplate wells, and the binding assay proceeded as described in Materials and Methods. Data are shown for strain 6594 and represent the mean and standard error of the mean of four replicates for each treatment.