Fig. 2.

IL-17A regulates growth of gastric epithelial cells. A Immunohistochemical analysis of proliferating cell nuclear antigen (PCNA) of gastric tissues of WT and IL-17A KO mice (original magnification = ×200). Ten random fields of each sample were selected and positive cell in each image were counted. B Immunoblot analysis of cyclin D1 in WT and IL-17A KO mice. Expression of cyclin D1 is normalized to that of β-actin. C Representative photomicrographs of TUNEL staining in gastric tissues of WT and IL-17A KO mice. Boxed regions of left panels (original magnification = ×200) are shown at higher magnification (original magnification = ×400) in the right panels. Ten fields were randomly selected for the quantification of positive cells per slide. D Cell cycle analysis. AGS cells were treated with rhIL-17A (50 ng/ml) for 12 h and changes in the percentage of G1 phase of cells were measured via propidium iodide (PI) staining using flow cytometry. E Analysis of cell apoptosis. The degree of apoptosis was assessed via flow cytometry using Annexin V and PI staining. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01 versus WT or vehicle; ^#P < 0.05, ^##P < 0.01 versus same genotype control