Figure 4.

Ot infected dendritic cells and macrophages, which migrated to draining lymph nodes in mice. B6 mice (n=4/group) were i.d. infected with CFSE-labeled Ot (4×10³ FFU) in the ears. dLN were harvested at indicated time-points, and prepared for single cell suspension, followed by flow cytometric analysis. (A) Live cells were sorted by live/dead dye staining, and Ot-infected immune cells were identified by expression of CD45⁺CFSE⁺. We determined that Ot infected the following immune cell subpopulations: Langerhans cells (MHCII^hiCD24^hiEpCAM⁺), macrophages (MHCII⁺CD24^hiCD64⁺EpCAM^-), conventional dendritic cells (MHCII^hiCD24^hiCD64^-EpCAM^-), neutrophils (MHCII^-CD24^-Ly6G⁺), and Ly6C^hi monocytes (MHCII^-CD24^-Ly6G^-Ly6C^hiCCR2^hi). (B) The absolute Ot-infected cell numbers in dLN were calculated. Values are shown as mean ± SEM from single experiments and are representative of two independent experiments. A one-way ANOVA with Tukey’s multiple comparisons test was used for statistical analysis. *, p<0.05; **, p<0.01; ***, p <0.001.