Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jan 4;6:8. doi: 10.1038/s42003-022-04404-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a Representative 100x immunohistochemical images of PEX14 labeled (green) and DAPI stained (blue) 3-month-old WT, Ppara^−/−, Tmem135^{FUN025/FUN025} (FUN025), and Tmem135^{FUN025/FUN025}/Ppara^−/− (FUN025/Ppara^−/−) livers. The white boxes in these images were expanded to highlight differences of the PEX14-positive peroxisome staining between these genotypes. Scale bar for images = 50 microns. b Quantitation of PEX14-positive peroxisomes from the 100x images of WT (1 male/ 2 females), Ppara^−/− (1 male/2 females), Tmem135^{FUN025/FUN025} (1 male/2 females), and Tmem135^{FUN025/FUN025}/Ppara^−/− (1 male/ 3 females) livers using the Analyze Particles function in ImageJ. c Western blot analysis of peroxisomal proteins in the livers of 3-month-old WT (3 males/1 female), Ppara^−/− (2 males/2 females), Tmem135^{FUN025/FUN025} (2 males/2 females), and Tmem135^{FUN025/FUN025}/Ppara^−/− (3 males/2 females) mice. HSC70 served as the loading control for these experiments. Asterisks (*, **, ***, and ****) indicates post hoc Tukey test for a P < 0.05, P < 0.01, P < 0.001, and P < 0.0001 significance following a significant difference detected by one-way ANOVA. PMP70, peroxisomal membrane protein 70. ACOX1, acyl-CoA oxidase 1. DBP, D-bifunctional protein. ACAA1, acetyl-Coenzyme A acyltransferase 1. Dots represent individual data points. Number in parentheses represents N of independent mouse samples per genotype used in the experiment. The protein size next to the immunoblot images denotes the size of the immunoband measured for this analysis. Data are presented as mean ± SD.