Fig. 2. Evaluation for the orthosteric ligand-binding sites.

Ligands binding to the orthosteric sites in experimental structures and predicted models of 5-hydroxytryptamine receptor 1F receptor (5HT_1FR) (a), melatonin receptor 1A (MT₁R) (b), and luteinizing hormone and choriogonadotropin receptor (LHCGR) (c). Cryo-EM structures of 5HT_1FR (PDB ID: 7EXD), MT₁R (PDB ID: 7DB6), LHCGR (PDB ID: 7FIH) are selected for analysis. Key residues and ligands are shown as sticks. Pink, blue, orange, and green represent experimental structures, predicted models, ligands from experimental complex structures, and docked ligands, respectively. The ligand-binding pockets are shown as surfaces.