Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jan 4;14:61. doi: 10.1038/s41467-022-35725-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a Electrostatic surface representation of NTD-GSDMB. The basic residues are colored in blue and shown in stick representations. b Cell death was assessed by LDH cytotoxicity assay in HEK293T cells. Mutations of the basic residues in positively charged regions decreased the NTD-mediated LDH release. All experiments were repeated at least three times (mean ± sd, n = 3 independent biological replicates). Bottom, the expression levels of GSDMB proteins. c Pore model of NTD-GSDMB. The residues in the oligomerization interface selected for mutation are shown in pink sphere representations. d Mutations of the residues in the oligomerization interface reduced the NTD-mediated LDH release. All experiments were repeated at least three times (mean ± sd, n = 3 independent biological replicates). Bottom, the expression levels of GSDMB proteins. Source data are provided as a Source Data file.