Fig. 2. Aspirin decreases the levels of PGAM1 Succ in hepatoma cells.
a, b Total cell lysate from HepG2 and Huh7 cells treated with 4 mM aspirin was used to analyze the succinylation levels by using Western blot analysis with the indicated pan anti-succinylation antibody. Total protein levels were measured by Coomassie blue staining. c, d IP assays were performed by using PGAM1 antibody in HepG2 and Huh7 cells treated with 4 mM aspirin for 48 h. The levels of PGAM1 Succ were detected by Western blot analysis.