Table 1.

Determination of P450s activity in liver microsomes by HPLC–MS/MS.

P450 subtype		Control	HFD	Silybin (50 mg/kg)	Silybin (100 mg/kg)
CYP1A2 (Acetaminophen)	Vmax (μmol·min-1·g-1 protein)	0.244 ± 0.006	0.191 ± 0.014	0.213 ± 0.012	0.127 ± 0.007
	CLint (mL·min-1·g-1 protein)	2.071	1.048	1.694	1.164
CYP2B6 (Hydroxybupropion)	Vmax (μmol·min-1·g-1 protein)	0.024 ± 0.001	0.016 ± 0.001	0.032 ± 0.006	0.028 ± 0.002
	CLint (mL·min-1·g-1 protein)	0.057	0.030	0.035	0.039
CYP2C19 (Hydroxyomeprazole)	Vmax (μmol·min-1·g-1 protein)	0.243 ± 0.179	0.113 ± 0.030	0.102 ± 0.027	0.146 ± 0.012
	CLint (mL·min-1·g-1 protein)	1.873	1.463	1.688	3.628
CYP2D6 (Dextropan)	Vmax (μmol·min-1·g-1 protein)	0.111 ± 0.004	0.043 ± 0.002	0.056 ± 0.004	0.038 ± 0.004
	CLint (mL·min-1·g-1 protein)	22.82	15.95	21.73	10.07
CYP3A (4-hydroxymidazolam)	Vmax (μmol·min-1·g-1 protein)	0.290 ± 0.049	0.267 ± 0.042	0.267 ± 0.036	0.293 ± 0.077
	CLint (mL·min-1·g-1 protein)	3.756	2.935	3.470	4.741
CYP3A (6β-hydroxytestosterone)	Vmax (μmol·min-1·g-1 protein)	0.277 ± 0.077	0.147 ± 0.025	0.196 ± 0.022	0.187 ± 0.063
	CLint (mL·min-1·g-1 protein)	1.271	0.569	1.146	0.820