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. 2023 Jan 4;6:9. doi: 10.1038/s42003-022-04392-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Schematic of the experiment created in BioRender (https://biorender.com/) by F.S.d.C. as a registered user. b Percentage of cells able to complete mitosis following the round up; data are shown as mean ± s.e.m.; dots represent the mean for each experiment (n = 67 WT, 44 PFN1^+/−, 43 PFN1^−/− RPE1 cells; range of number of cells used for each individual experiment: 9-25 WT, 13-16 PFN1^+/−, 11-16 PFN1^−/−); ***P = 0.0005, **P = 0.0023. The mean difference between PFN1^+/− and PFN1^−/− cells was not statistically significant (P = 0.4310). c Time taken from start (cell rounding) to end (cytokinesis) of mitosis; data are shown as mean ± s.e.m.; dots represent the duration of each cell division expressed in minutes (n mitoses = 39 WT, 8 PFN1^+/−, 14 PFN1^−/− RPE1 cells); ****P < 0.0001. Data in b and c were analysed by ordinary one-way ANOVA. d Representative time-lapse phase-contrast images (taken every 2 min) of WT and e PFN1-KO RPE1 cells; scale bars 25 μm. Time stamps indicate elapsed time in hours:minutes. f WT, PFN1^+/− and PFN1^−/− RPE1 cells at metaphase stained for DNA (Hoechst 33342, blue), Profilin 1 (green) and F-actin (phalloidin, magenta); scale bars 5 μm. g Mean fluorescence intensity of cortical actin; data are shown as mean ± s.e.m.; dots represent the fluorescent intensity for each sample (n = 25 for WT, 17 for PFN1^+/−, 20 for PFN1^−/− cells); ***P = 0.0002, ****P < 0.0001, *P = 0.0436. Ordinary one-way ANOVA performed.