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Binding of mAbs to different synthetic MERS-CoV S2 peptides. (A) Diagram of MERS-CoV S protein. Amino acid (aa) positions are referred to the sequence of strain HCoV-EMC (GenBank: AFS88936.1). (B) Amino acid sequence of peptides. (C) Binding of mAbs MSP2-34, MSP6-25, and MSP6-31 (1 μg/ml) to synthetic peptides (20 ng/μl) by ELISA. MERS-CoV S2 subunit (2 μg/ml) was positive control. BSA was used as negative control.
