Table 1.
Genetic characteristics of mAbs with reactivity against MERS-COV S1 and/or S2.
| mAbs | MERS-CoV, EC50(μg/mL) | Binding domain | Heavy chain | Light chain | |||||
|---|---|---|---|---|---|---|---|---|---|
| S1 | S2 | VH gene | H-CDR3 (bp) | VH identify (%) | VL gene | L-CDR3 (bp) | VL identify (%) | ||
| MSP2-3 | 0.186 | 0.135 | S1 and S2 | IGHV3-74 | 8 | 89.2 | IGLV2-14 | 10 | 92.9 |
| MSP2-34 | – | 0.003 | S2 | IGHV1-46 | 13 | 91.1 | IGKV1-27 | 11 | 94.1 |
| MSP2-36 | – | 0.007 | S2 | IGHV4-59 | 19 | 86.4 | IGLV2-8 | 10 | 96.9 |
| MSP4-33 | – | 0.292 | S2 | IGHV2-5 | 15 | 90 | IGKV4-1 | 9 | 86.7 |
| MSP5-44 | – | 0.014 | S2 | IGHV3-11 | 20 | 92.5 | IGKV3-11 | 11 | 90.9 |
| MSP5-45 | – | 8.810 | S2 | IGHV3-7 | 16 | 91.6 | IGKV2-30 | 9 | 95.7 |
| MSP6-10 | – | 0.516 | S2 | IGHV3-7 | 16 | 89.2 | IGKV3-20 | 9 | 89.8 |
| MSP6-19 | – | 0.366 | S2 | IGHV3-48 | 12 | 88.2 | IGLV1-44 | 11 | 92.8 |
| MSP6-25 | 0.788 | 0.241 | S1 and S2 | IGHV3-11 | 19 | 97.6 | IGLV2-23 | 13 | 96.2 |
| MSP6-31 | – | 0.004 | S2 | IGHV3-30 | 12 | 94.6 | IGKV1-9 | 9 | 95.1 |
| MSP6-40 | – | 2.453 | S2 | IGHV3-7 | 16 | 95.6 | IGKV2-30 | 9 | 95.3 |
ELISA-based half-maximal effective concentrations (EC50) and genetic characterization of mAbs. Heavy and light chain genes, % difference relative to germline sequence and CDR3 length were analyzed using the IgBlast website (http://www.ncbi.nlm.nih.gov/igblast/).