Figure 2.

Immunostaining, western blotting and reverse transcription-quantitative PCR analysis of target protein and mRNA expression levels after transfection. (A) Protein expression levels of cTnI in BMSCs were assessed using immunostaining with cTnI-specific primary antibodies and a Cy3 secondary antibody. Scale bar, 50 µm. (B) Quantitative analysis of the relative mRNA expression levels of HCN4, α-SA and cTnI. (C) Western blotting demonstrated increased HCN4, α-SA and cTnI protein expression levels. BMSCs without transfection were used as the control and BMSCs transfected with the empty vector were used as the NC. **P<0.01. cTnI, cardiac troponin I; HCN4, hyperpolarization-activated cyclic nucleotide-gated channel 4; α-SA, α-striated actin; NC, negative control; CM, cardiomyocyte; BMSCs, bone marrow mesenchymal stem cells.