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Proteomic analysis of aging C. elegans at different temperatures (a) Schematic overview of proteome analysis. Synchronized worms at different stages were lysed and mixed with iTRAQ-labeled peptides. After separation into 12 fractions, the peptides were analyzed by a nano HPLC system coupled to ESI-MS. (b) Venn diagrams of the shared and distinctive proteins identified from three biological replicates. (c) The Principal Component Analysis (PCA) map of three replicates.
