Fig. 5.

The regime of high temperature that extends the lifespan was temporally restricted (a) The temporal effects of high-temperature shift on the lifespan. Animals were shifted from 20 °C to 25 °C at specific stages (late-L4, Day 1, Day 2, Day 3, Day 4) for 24 h then back to 20 °C, followed by the performance of adult lifespan assay (late-L4: p < 0.001; Day 1: p = 0.01; Day 2: p = 0.023; Day 3: p = 0.369; Day 4: p = 0.826; log-rank test). (b) The optimal high-temperature duration is required for the lifespan extension. Animals were shifted from 20 °C to 25 °C for different period between 3 h and 5 days starting from the L4 stage ( 3 h from L4: p = 0.268; 6 h from L4: p = 0.292; 12 h from L4: p = 0.085; 18 h from L4: p = 0.032; 1 day from L4: p < 0.001; 2 days from L4: p = 0.233; 3 days from L4: p = 0.354; 4 days from L4: p = 0.848; 5 days from L4: p = 0.151; log-rank test). (c) High-temperature regime promotes stress resistance. Animals in the high-temperature regime were more resistant to oxidative stress (p < 0.001; log-rank test), heat stress (p < 0.001; log-rank test), and UV light (p < 0.001; log-rank test).